Journal: Frontiers in Immunology

Article Title: 29-Color Flow Cytometry: Unraveling Human Liver NK Cell Repertoire Diversity

doi: 10.3389/fimmu.2019.02692

Figure Lengend Snippet: Antibodies used in this study.

Article Snippet: CD49e , REA686 , VioBright FITC , , 530/30 , 100 , No , Miltenyi biotec , Tissue residency/cell retention.

Techniques: Marker, Staining

Journal: Frontiers in Immunology

Article Title: 29-Color Flow Cytometry: Unraveling Human Liver NK Cell Repertoire Diversity

doi: 10.3389/fimmu.2019.02692

Figure Lengend Snippet: A 29-color NK-cell focused panel captures phenotypic diversity within liver-enriched NK cells. (A) Heatmap displays Z-score transformed median expression values for each of the parameters within 18 identified PhenoGraph clusters as described in . Color scale was determined for each column separately, based on the lowest and highest Z-score value of that parameter. (B) Histograms displaying expression levels of selected proteins in PhenoGraph clusters. Legend indicates whether a cluster is predominantly enriched in liver (#3), present in similar frequencies in liver and blood (shared, #18) or enriched in blood (#10, #12). (C) Histograms displaying expression levels of selected proteins from (A) in selected liver-enriched clusters (#4, #5, #15). (D) Identification of the two most proliferating PhenoGraph clusters (#3 and #14) according to their Ki-67 expression level based on analysis in (A) , displayed against CD49e.

Article Snippet: CD49e , REA686 , VioBright FITC , , 530/30 , 100 , No , Miltenyi biotec , Tissue residency/cell retention.

Techniques: Transformation Assay, Expressing

Journal: Gut Pathogens

Article Title: Hypoxia exacerbates intestinal injury and inflammatory response mediated by myeloperoxidase during Salmonella Typhimurium infection in mice

doi: 10.1186/s13099-023-00586-5

Figure Lengend Snippet: Hypoxia exacerbates bacterial translocation in S. Typhimurium-infected mice. Bacterial translocation to the spleen ( A ) and liver ( B ) was determined 72 h after infection via one-way ANOVA with Tukey’s multiple comparison test. The CFU number per gram of tissue was displayed (n = 8–10/group; * p < 0.05; ** p < 0.01; *** p < 0.001). ( C ) Immunofluorescence microscopy revealed the presence of Salmonella (in green) in spleen sections (×200). The spleen ( D ) and liver ( E ) were stained with hematoxylin and eosin [( D ) ×200; ( E ) ×400]. The blue arrow represents lymphocyte hyperplasia. The black arrow means red marrow congestion. The yellow arrow indicates that the structure of the liver plate is unclear. The green arrow represents focal abscess. ( F – I ) Relative mRNA expression of TNF-α ( F , H ) and IL-1β ( G , I ) in the spleen and liver tissues was detected by qPCR. ( J - M ) Levels of TNF-α ( J , L ) and IL-1β ( K , M ) were detected by ELISA. Data are presented as mean ± SEM (n = 8 /group) and analyzed via one-way ANOVA with Tukey’s multiple comparisons test (* p < 0.05; ** p < 0.01; *** p < 0.001)

Article Snippet: ELISA capture antibodies (BD Cat. #557,516) and biotinylated secondary antibodies for TNF-α (BD Cat. #558,415), and standard curve was constructed using recombinant murine TNF-α (BD Cat. #554,589) antibodies purchased from BD Biosciences (Franklin Lakes, NJ, USA).

Techniques: Translocation Assay, Infection, Comparison, Immunofluorescence, Microscopy, Staining, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Gut Pathogens

Article Title: Hypoxia exacerbates intestinal injury and inflammatory response mediated by myeloperoxidase during Salmonella Typhimurium infection in mice

doi: 10.1186/s13099-023-00586-5

Figure Lengend Snippet: Hypoxia augments Salmonella -induced mucosal injury and colitis in mice. ( A ) Hematoxylin and eosin staining of the colon (×100, ×400). ( B ) Immunofluorescence microscopy data show the distribution of Salmonella (in green) in colon sections (×200). Levels of TNF-α ( C ) and IL-1β ( D ) were determined by quantitative PCR. Cytokine levels of TNF-α ( E ) and IL-1β ( F ) were detected by ELISA. Colon levels of KC ( G ), MCP1 ( H ), and MIP2 ( I ) were analyzed by qPCR. Data represent mean ± SEM (n = 8 /group) and one-way ANOVA followed by Tukey’s multiple comparisons test (* p < 0.05; ** p < 0.01; *** p < 0.001)

Article Snippet: ELISA capture antibodies (BD Cat. #557,516) and biotinylated secondary antibodies for TNF-α (BD Cat. #558,415), and standard curve was constructed using recombinant murine TNF-α (BD Cat. #554,589) antibodies purchased from BD Biosciences (Franklin Lakes, NJ, USA).

Techniques: Staining, Immunofluorescence, Microscopy, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay